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pCAS-mak10 gRNA-MAK10Δ
(Plasmid #160384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAS-mak10 gRNA
  • Backbone size w/o insert (bp) 8743
  • Total vector size (bp) 9714
  • Vector type
    Yeast Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAK10
  • Alt name
    YEL053C
  • Species
    S. cerevisiae (budding yeast)
  • Promoter PGK1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MAK10 was deleted in an S288C background with Cre-loxP. Lox-ed mak10 "deletion " fragment" consisting of sequences upstream and downstream of deleted ORF (~800bp) was amplified, adding XhoI restriction sites, and cloned into the vector. This insert serves as a donor template for making a MAK10 deletion. The vector is an all-in-one gRNA/Cas9/donor template vector for making a marker-free MAK10 deletion in yeast strains

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAS-mak10 gRNA-MAK10Δ was a gift from John McCusker (Addgene plasmid # 160384 ; http://n2t.net/addgene:160384 ; RRID:Addgene_160384)
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