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Addgene

pGAL-SKI1-NAT
(Plasmid #160382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLND46
  • Backbone manufacturer
    Genetics. 2009 Sep; 183(1): 365–383.
  • Backbone size w/o insert (bp) 5643
  • Total vector size (bp) 10245
  • Vector type
    Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SKI1
  • Alt name
    XRN1
  • Alt name
    YGL173C
  • Alt name
    KEM1, DST2, RAR5 , SEP1
  • Species
    S. cerevisiae (budding yeast)
  • Promoter GAL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAL-SKI1-NAT was a gift from John McCusker (Addgene plasmid # 160382 ; http://n2t.net/addgene:160382 ; RRID:Addgene_160382)
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