pRK5 beta4 1355T
(Plasmid #16038)

• Depositing Lab: Filippo Giancotti
• Publication: Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-102.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta4
• Alt name: beta 4 integrin
• Species: H. sapiens (human)
• Insert Size (bp): 4100
• Mutation: 1355T: amino acids 1-1355
• Entrez Gene: ITGB4 (a.k.a. CD104, GP150, JEB5A, JEB5B)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry