pDule2-Mb haloTyrRS C6
(Plasmid
#160378)
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PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160378 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDule
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Vector typeMammalian Expression, Bacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameC6 HaloTyrosine tRNA synthatase
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SpeciesSynthetic; Methanosarcina barkeri
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MutationL270S, Y271L, N311G, C313T
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDule2-Mb haloTyrRS C6 was a gift from Ryan Mehl (Addgene plasmid # 160378 ; http://n2t.net/addgene:160378 ; RRID:Addgene_160378) -
For your References section:
Efficient Site-Specific Prokaryotic and Eukaryotic Incorporation of Halotyrosine Amino Acids into Proteins. Jang HS, Gu X, Cooley RB, Porter JJ, Henson RL, Willi T, DiDonato JA, Hazen SL, Mehl RA. ACS Chem Biol. 2020 Feb 21;15(2):562-574. doi: 10.1021/acschembio.9b01026. Epub 2020 Feb 10. 10.1021/acschembio.9b01026 PubMed 31994864