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Addgene

pDule-Mb haloTyrRS C6
(Plasmid #160377)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDule
  • Vector type
    Mammalian Expression, Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    C6 HaloTyrosine tRNA synthatase
  • Species
    Synthetic; Methanosarcina barkeri
  • Mutation
    L270S, Y271L, N311G, C313T

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDule-Mb haloTyrRS C6 was a gift from Ryan Mehl (Addgene plasmid # 160377 ; http://n2t.net/addgene:160377 ; RRID:Addgene_160377)

  • For your References section:

    Efficient Site-Specific Prokaryotic and Eukaryotic Incorporation of Halotyrosine Amino Acids into Proteins. Jang HS, Gu X, Cooley RB, Porter JJ, Henson RL, Willi T, DiDonato JA, Hazen SL, Mehl RA. ACS Chem Biol. 2020 Feb 21;15(2):562-574. doi: 10.1021/acschembio.9b01026. Epub 2020 Feb 10. 10.1021/acschembio.9b01026 PubMed 31994864
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