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Addgene

MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)
(Plasmid #160369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJC5
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPR143
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Entrez Gene
    GPR143 (a.k.a. NYS6, OA1)
  • Tag / Fusion Protein
    • mScarlet-3xHA (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA) was a gift from David Sabatini (Addgene plasmid # 160369 ; http://n2t.net/addgene:160369 ; RRID:Addgene_160369)

  • For your References section:

    MFSD12 mediates the import of cysteine into melanosomes and lysosomes. Adelmann CH, Traunbauer AK, Chen B, Condon KJ, Chan SH, Kunchok T, Lewis CA, Sabatini DM. Nature. 2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 10.1038/s41586-020-2937-x. 10.1038/s41586-020-2937-x PubMed 33208952
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