pRK5 alpha6
(Plasmid #16036)

• Depositing Lab: Filippo Giancotti
• Publication: Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-502.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha6
• Alt name: alpha 6 integrin
• Species: H. sapiens (human)
• GenBank ID: NM_000210.2
• Entrez Gene: ITGA6 (a.k.a. CD49f, ITGA6A, ITGA6B, JEB6, VLA-6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results indicate that this plasmid contains alpha-6 integrin isoform b.