pQE80L-SH3
(Plasmid #160331)

• Purpose: This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.
• Depositing Lab: Sang Taek Jung
• Publication: Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12037-12043. doi: 10.1021/acs.jafc.9b05473. Epub 2019 Oct 17.

Backbone

• Vector backbone: pQE81L
• Backbone manufacturer: Qiagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SH3
• Species: Engineered sequence derived from Arabidopsis blue light receptor LOV domain gene
• Insert Size (bp): 330
• Promoter: T5
• Tag / Fusion Protein:
  • 6xHis tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: 5'-CGGAT AACAA TTTCA CACAG-3'
• 3′ sequencing primer: 3'-GGTCA TTACT GGAGT CTTG-5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry