p7SKU6SBpur
(Plasmid
#160329)
-
Purpose(Empty Backbone) dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 160329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSilencer 1.0-U6
-
Backbone manufacturerAmbion
-
Vector typeMammalian Expression
-
Selectable markersPuromycin
-
Tag
/ Fusion Protein
- cfSBFP2
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p7SKU6SBpur was a gift from Ikuo Wada (Addgene plasmid # 160329 ; http://n2t.net/addgene:160329 ; RRID:Addgene_160329) -
For your References section:
Visualization of Procollagen IV Reveals ER-to-Golgi Transport by ERGIC-independent Carriers. Matsui Y, Hirata Y, Wada I, Hosokawa N. Cell Struct Funct. 2020 Jul 23;45(2):107-119. doi: 10.1247/csf.20025. Epub 2020 Jun 18. 10.1247/csf.20025 PubMed 32554938