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Addgene

cfSGFP2-col4a1
(Plasmid #160317)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160317 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    EGFP-N1
  • Backbone manufacturer
    Clonetech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COL4A1
  • Species
    H. sapiens (human)
  • Entrez Gene
    COL4A1 (a.k.a. BSVD, BSVD1, COL4A1s, PADMAL, RATOR)
  • Tag / Fusion Protein
    • cfSGFP2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a T824P mutation in the insert (lies within the Mature Domain). This variant is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cfSGFP2-col4a1 was a gift from Ikuo Wada (Addgene plasmid # 160317 ; http://n2t.net/addgene:160317 ; RRID:Addgene_160317)
  • For your References section:

    Visualization of Procollagen IV Reveals ER-to-Golgi Transport by ERGIC-independent Carriers. Matsui Y, Hirata Y, Wada I, Hosokawa N. Cell Struct Funct. 2020 Jul 23;45(2):107-119. doi: 10.1247/csf.20025. Epub 2020 Jun 18. 10.1247/csf.20025 PubMed 32554938