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Addgene

59 pLIB_HIS_HA
(Plasmid #160308)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160308 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIB
  • Modifications to backbone
    MCS altered for full compatibility within InteBac, C-terminal 3C-cleavable 12xHis-3xHA tag added
  • Vector type
    Insect Expression
  • Promoter polyhydrin
  • Selectable markers
    Gentamicin
  • Tags / Fusion Proteins
    • 12xHis (N terminal on backbone)
    • 3xHA (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    59 pLIB_HIS_HA was a gift from John Weir (Addgene plasmid # 160308 ; http://n2t.net/addgene:160308 ; RRID:Addgene_160308)

  • For your References section:

    InteBac - An integrated bacterial and baculovirus expression vector suite. Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR. Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957. 10.1002/pro.3957 PubMed 32955754
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