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Addgene

pCY5h
(Plasmid #160295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAS (Addgene #60847)
  • Backbone manufacturer
    Jamie Cate
  • Total vector size (bp) 10151
  • Vector type
    CRISPR
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
  • Species
    Synthetic
  • Insert Size (bp)
    379

Gene/Insert 2

  • Gene/Insert name
    URA3 selection cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1385

Gene/Insert 3

  • Gene/Insert name
    sgRNA expression cassette (without guide)
  • Species
    Synthetic
  • Insert Size (bp)
    365

Gene/Insert 4

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4176
  • Tags / Fusion Proteins
    • SV40 NLS (C terminal on insert)
    • 8xHis-tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCY5h was a gift from Amir Aharoni (Addgene plasmid # 160295 ; http://n2t.net/addgene:160295 ; RRID:Addgene_160295)

  • For your References section:

    Marker-free genetic manipulations in yeast using CRISPR/CAS9 system. Soreanu I, Hendler A, Dahan D, Dovrat D, Aharoni A. Curr Genet. 2018 Oct;64(5):1129-1139. doi: 10.1007/s00294-018-0831-y. Epub 2018 Apr 6. 10.1007/s00294-018-0831-y PubMed 29626221
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