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Addgene

SGyA
(Plasmid #160292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    attB
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 16663
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Vas promoter
  • Alt name
    Vasa promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2241

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    hSpCas9
  • Alt name
    Cas9
  • Insert Size (bp)
    5043
  • Tag / Fusion Protein
    • T2A GFP (C terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    tdTomato
  • Alt name
    tdTomato

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    CTCF insulator
  • Alt name
    CTCF insulator
  • Insert Size (bp)
    308

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    Gypsy insulator
  • Alt name
    Gypsy insulator
  • Insert Size (bp)
    432

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    Left homology arm Y chromosome
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    811

Cloning Information for Gene/Insert 6

Gene/Insert 7

  • Gene/Insert name
    Right homology arm Y chromosome
  • Insert Size (bp)
    813

Cloning Information for Gene/Insert 7

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SGyA was a gift from Omar Akbari (Addgene plasmid # 160292 ; http://n2t.net/addgene:160292 ; RRID:Addgene_160292)

  • For your References section:

    Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive. Gamez S, Chaverra-Rodriguez D, Buchman A, Kandul NP, Mendez-Sanchez SC, Bennett JB, Sanchez C HM, Yang T, Antoshechkin I, Duque JE, Papathanos PA, Marshall JM, Akbari OS. Nat Commun. 2021 Dec 10;12(1):7202. doi: 10.1038/s41467-021-27333-1. 10.1038/s41467-021-27333-1 PubMed 34893590
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