pYM29-BirA*
(Plasmid
#160291)
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PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160291 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYM29
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBirA*
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYM29-BirA* was a gift from Martin Ott (Addgene plasmid # 160291 ; http://n2t.net/addgene:160291 ; RRID:Addgene_160291) -
For your References section:
Molecular Wiring of a Mitochondrial Translational Feedback Loop. Salvatori R, Kehrein K, Singh AP, Aftab W, Moller-Hergt BV, Forne I, Imhof A, Ott M. Mol Cell. 2020 Feb 20;77(4):887-900.e5. doi: 10.1016/j.molcel.2019.11.019. Epub 2019 Dec 26. 10.1016/j.molcel.2019.11.019 PubMed 31883951