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pLEX307-Bat-CoV-HKU9-3CL
(Plasmid #160284)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160284 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX_307
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable ; NEB 10Beta
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3CL Protease
  • Species
    Bat-CoV-HKU9
  • Insert Size (bp)
    924
  • Promoter EF1a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is stored and distributed in NEB Stable. It can also be used to transform NEB 10Beta.

The authors have noticed that there are high rates of recombination in these generated vectors. All users are advised to sequence the vectors and run them on a gel to ensure they show the expected full length insert and plasmid size. In cases where recombination has occurred it is typically between the viral LTR elements in the vector although recombination between the gateway cloning sites has also been observed.

Please visit https://doi.org/10.1101/2020.08.29.272864 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307-Bat-CoV-HKU9-3CL was a gift from Alejandro Chavez (Addgene plasmid # 160284 ; http://n2t.net/addgene:160284 ; RRID:Addgene_160284)

  • For your References section:

    Inhibitors of Coronavirus 3CL Proteases Protect Cells from Protease-Mediated Cytotoxicity. Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, Stockwell BR, Ho DD, Chavez A. J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. Epub 2021 Jun 24. 10.1128/JVI.02374-20 PubMed 33910954
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