pLEX307-MERS-CoV-3CL-C148A
(Plasmid
#160283)
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PurposeAllows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEX_307
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable ; NEB 10Beta
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3CL Protease
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SpeciesMERS-CoV
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Insert Size (bp)924
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MutationC148A
- Promoter EF1a
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
bioRxiv. 2020 Aug 29. doi: 10.1101/2020.08.29.272864 This plasmid is stored and distributed in NEB Stable. It can also be used to transform NEB 10Beta.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307-MERS-CoV-3CL-C148A was a gift from Alejandro Chavez (Addgene plasmid # 160283 ; http://n2t.net/addgene:160283 ; RRID:Addgene_160283) -
For your References section:
Inhibitors of Coronavirus 3CL Proteases Protect Cells from Protease-Mediated Cytotoxicity. Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, Stockwell BR, Ho DD, Chavez A. J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. Epub 2021 Jun 24. 10.1128/JVI.02374-20 PubMed 33910954