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4 pCOLI_A_3c_C_HIS
(Plasmid #160243)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160243 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST50Trc
  • Modifications to backbone
    Custom MCS added to the vector for full compatibility within the InteBac system. C-terminal 3C cleavable 8xHis tagged added.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • 8xHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearise vector with BamHI and HindIII, clone gene of interest in using COLI_Fwd and Tag_Rev primers using Gibson assembly, according to InteBac suite instructions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4 pCOLI_A_3c_C_HIS was a gift from John Weir (Addgene plasmid # 160243 ; http://n2t.net/addgene:160243 ; RRID:Addgene_160243)

  • For your References section:

    InteBac - An integrated bacterial and baculovirus expression vector suite. Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR. Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957. 10.1002/pro.3957 PubMed 32955754
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