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Addgene

908H
(Plasmid #160240)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    attB
  • Backbone size w/o insert (bp) 7400
  • Total vector size (bp) 14000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Tet Operator (2x)
  • Alt name
    TetO
  • Insert Size (bp)
    55

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    hsp70 minimal promoter
  • Alt name
    hsp70 minimal promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    130

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    p-element promoter
  • Alt name
    p-element promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    249

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    Firefly luciferase
  • Insert Size (bp)
    2424
  • Tag / Fusion Protein
    • T2A GFP (C terminal on insert)

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    Renilla luciferase
  • Alt name
    Renilla luciferase
  • Insert Size (bp)
    936

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    Ubiquitin promoter
  • Alt name
    Ubiquitin promoter
  • Insert Size (bp)
    1869

Cloning Information for Gene/Insert 6

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    908H was a gift from Omar Akbari (Addgene plasmid # 160240 ; http://n2t.net/addgene:160240 ; RRID:Addgene_160240)

  • For your References section:

    Spatial control of gene expression in flies using bacterially derived binary transactivation systems. Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS. Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. Epub 2021 May 24. 10.1111/imb.12717 PubMed 33963794
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