907F1
(Plasmid
#160233)
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PurposeFlightN-VanR-VP16-3X-SV40-attB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 160233 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneattB
- Backbone size w/o insert (bp) 8100
- Total vector size (bp) 9576
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameVanR
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SpeciesCaulobacter crescentus
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Insert Size (bp)735
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Tag
/ Fusion Protein
- VP16 (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameFlightin promoter
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Alt nameFln
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SpeciesD. melanogaster (fly)
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Insert Size (bp)572
Cloning Information for Gene/Insert 2
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
907F1 was a gift from Omar Akbari (Addgene plasmid # 160233 ; http://n2t.net/addgene:160233 ; RRID:Addgene_160233) -
For your References section:
Spatial control of gene expression in flies using bacterially derived binary transactivation systems. Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS. Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. Epub 2021 May 24. 10.1111/imb.12717 PubMed 33963794