pMMLV-EBFP-P2A-Puro
(Plasmid
#160227)
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PurposeExpresses EBFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMMLV
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Backbone manufacturerVectorBuilder
- Backbone size w/o insert (bp) 4812
- Total vector size (bp) 6195
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Vector typeMammalian Expression, Bacterial Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEBFP-P2A-Puro
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SpeciesSynthetic
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Insert Size (bp)1383
- Promoter LTR
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-N
- 3′ sequencing primer Puro-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMMLV-EBFP-P2A-Puro was a gift from Jason Sheltzer (Addgene plasmid # 160227 ; http://n2t.net/addgene:160227 ; RRID:Addgene_160227) -
For your References section:
Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies. Lukow DA, Sausville EL, Suri P, Chunduri NK, Wieland A, Leu J, Smith JC, Girish V, Kumar AA, Kendall J, Wang Z, Storchova Z, Sheltzer JM. Dev Cell. 2021 Sep 13;56(17):2427-2439.e4. doi: 10.1016/j.devcel.2021.07.009. Epub 2021 Aug 4. 10.1016/j.devcel.2021.07.009 PubMed 34352222