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Addgene

pMMLV-EGFP-P2A-Puro
(Plasmid #160226)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160226 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMMLV
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 4812
  • Total vector size (bp) 6195
  • Vector type
    Mammalian Expression, Bacterial Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-P2A-PuroR
  • Species
    Synthetic
  • Insert Size (bp)
    1383
  • Promoter LTR

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMLV-EGFP-P2A-Puro was a gift from Jason Sheltzer (Addgene plasmid # 160226 ; http://n2t.net/addgene:160226 ; RRID:Addgene_160226)
  • For your References section:

    Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies. Lukow DA, Sausville EL, Suri P, Chunduri NK, Wieland A, Leu J, Smith JC, Girish V, Kumar AA, Kendall J, Wang Z, Storchova Z, Sheltzer JM. Dev Cell. 2021 Sep 13;56(17):2427-2439.e4. doi: 10.1016/j.devcel.2021.07.009. Epub 2021 Aug 4. 10.1016/j.devcel.2021.07.009 PubMed 34352222