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pICH47811-pAtAct2::Cas9-nls::tSlATPase
(Plasmid #160221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47811
  • Backbone size w/o insert (bp) 4968
  • Total vector size (bp) 9920
  • Vector type
    Plant Expression, Synthetic Biology ; MoClo toolkit level 1 acceptor position 2 reverse

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pAtACT2::Cas9-nls::tSlATPase
  • Insert Size (bp)
    5548

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BSA1 (destroyed during cloning)
  • 3′ cloning site BSA1 (destroyed during cloning)
  • 5′ sequencing primer ggagtcgacaaaatttagaacgaactta
  • 3′ sequencing primer agacagtgatctcttgagttttat
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICH47811-pAtAct2::Cas9-nls::tSlATPase was a gift from Matias Kirst (Addgene plasmid # 160221 ; http://n2t.net/addgene:160221 ; RRID:Addgene_160221)

  • For your References section:

    Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula x alba. Triozzi P, Schmidt HW, Dervinis C, Kirst M, Conde D. Tree Physiol. 2021 May 7. pii: 6270869. doi: 10.1093/treephys/tpab066. 10.1093/treephys/tpab066 PubMed 33960379
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