α‐eGFP
(Plasmid #160214)

• Purpose: Retroviral construct to express an eGFP-tagged version of the α subunit of AP2
• Depositing Lab: Sandra Schmid
• Publication: Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10.1111/tra.12755. Epub 2020 Jul 22.

Backbone

• Vector backbone: pMIB
• Backbone manufacturer: Modified from pMIG
• Backbone size w/o insert (bp): 5000
• Modifications to backbone: BFP and IRES sequences removed
• Vector type: Retroviral
• Selectable markers: eGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: α‐eGFP
• Alt name: α‐eGFP-Adaptin
• Alt name: AP2A1/AP2A2
• Species: M. musculus (mouse)
• Insert Size (bp): 3687
• Mutation: Brain-specific Insert
• Entrez Gene: Ap2a2 (a.k.a. 2410074K14Rik, AF006990, AW146353, Adt, Adtab, C78001, L2, L25)
• Entrez Gene: Ap2a1 (a.k.a. Ad, Adtaa)
• Tag / Fusion Protein:
  • eGFP at Hinge region

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M.S. Robinson

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry