34 pCOLI_K_His
(Plasmid
#160177)
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Purpose(Empty Backbone) N-term 6xHis 3C cleavable Kan resistant bacterial expression vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSF
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Modifications to backboneCustom MCS added to the vector for full compatibility within the InteBac system. 3C cleavable 6xHis- fusion added to N-terminus.
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7_fwd
- 3′ sequencing primer T7_rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
34 pCOLI_K_His was a gift from John Weir (Addgene plasmid # 160177 ; http://n2t.net/addgene:160177 ; RRID:Addgene_160177) -
For your References section:
InteBac - An integrated bacterial and baculovirus expression vector suite. Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR. Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957. 10.1002/pro.3957 PubMed 32955754
