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Addgene

29 pCOLI_S_C_STREP
(Plasmid #160171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF
  • Modifications to backbone
    Custom MCS added to the vector for full compatibility within the InteBac system. Non-cleavable twin StrepII fusion added to C-terminus.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • 2xStrepII (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    29 pCOLI_S_C_STREP was a gift from John Weir (Addgene plasmid # 160171 ; http://n2t.net/addgene:160171 ; RRID:Addgene_160171)

  • For your References section:

    InteBac - An integrated bacterial and baculovirus expression vector suite. Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR. Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957. 10.1002/pro.3957 PubMed 32955754
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