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Mouse CRISPR Metabolic Gene Knockout Library
(Pooled Library #160129)

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  • Purpose

    This lentiviral CRISPR knockout library targets mouse metabolic genes, including most metabolic enzymes and transporters.

  • Vector Backbone

    LentiCRISPRv2-Opti (Plasmid #163126) - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 160129 gRNA pooled library 1 $430 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Mouse
  • Genes targeted
    2,865
  • gRNAs
    22,909
  • Controls
    50 guides targeting intergenic regions
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse CRISPR Metabolic Gene Knockout library was a gift from Kivanc Birsoy (Addgene #160129)

  • For your References section:

    Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells. Zhu XG, Chudnovskiy A, Baudrier L, Prizer B, Liu Y, Ostendorf BN, Yamaguchi N, Arab A, Tavora B, Timson R, Heissel S, de Stanchina E, Molina H, Victora GD, Goodarzi H, Birsoy K Cell Metab. 2020 Oct 28. pii: S1550-4131(20)30550-7. doi: 10.1016/j.cmet.2020.10.017. PubMed 33152324
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