pHpgRNA13
(Plasmid
#160127)
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PurposeTo express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160127 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPICZ A
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePTEF1-HH-gRNA-ADE2- HDV-TAMO
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gRNA/shRNA sequenceUnspecified
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHpgRNA13 was a gift from Yongjin Zhou (Addgene plasmid # 160127 ; http://n2t.net/addgene:160127 ; RRID:Addgene_160127) -
For your References section:
Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha. Gao J, Gao N, Zhai X, Zhou YJ. iScience. 2021 Feb 9;24(3):102168. doi: 10.1016/j.isci.2021.102168. eCollection 2021 Mar 19. 10.1016/j.isci.2021.102168 PubMed 33665582
