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Addgene

pRWJB004
(Plasmid #160112)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZ001
  • Backbone manufacturer
    Lampropoulos et al
  • Backbone size (bp) 4114
  • Modifications to backbone
    Cassettes of pro::pcoCas9 and At2S3::mcherry are engineered into the backbone; and the cassette of ccdB-Chloramphenicol is replaced with proLacZ::LacZ expressing unit; BamHI and NotI are inserted to change the promoter for driving Cas9
  • Vector type
    Bacterial Expression, Plant Expression, CRISPR
  • Promoter UBQ10
  • Selectable markers
    red fluorescence

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRWJB004 was a gift from Detlef Weigel (Addgene plasmid # 160112 ; http://n2t.net/addgene:160112 ; RRID:Addgene_160112)

  • For your References section:

    An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana. Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, Schwab R, Weigel D. Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. eCollection 2018. 10.1186/s13007-018-0330-7 PubMed 30083222
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