pDONR221_mmIrf1 (closed)
(Plasmid
#160097)
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PurposeExpresses murine Irf1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221 backbone
- Backbone size w/o insert (bp) 3811
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Vector typeMammalian Expression ; Gateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIrf1
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SpeciesM. musculus (mouse)
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Entrez GeneIrf1 (a.k.a. Irf-1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_mmIrf1 (closed) was a gift from Rizwan Haq (Addgene plasmid # 160097 ; http://n2t.net/addgene:160097 ; RRID:Addgene_160097) -
For your References section:
Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance to PD-1 blockade. Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R. Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 10.1158/2159-8290.CD-19-1416. 10.1158/2159-8290.CD-19-1416 PubMed 32371478