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Addgene

pJB06T
(Plasmid #16008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16008 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS404
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACT1pr-DsRed.T1.N1
  • Alt name
    ACT1pr-DsRed
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1200

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was made by James Bean (Rockefeller University)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB06T was a gift from Fred Cross (Addgene plasmid # 16008 ; http://n2t.net/addgene:16008 ; RRID:Addgene_16008)

  • For your References section:

    The effects of molecular noise and size control on variability in the budding yeast cell cycle. Di Talia S, Skotheim JM, Bean JM, Siggia ED, Cross FR. Nature. 2007 Aug 23. 448(7156):947-51. 10.1038/nature06072 PubMed 17713537
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