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Addgene

pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine
(Plasmid #160069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2
  • Backbone manufacturer
    Modified from Bryan Roth (Addgene plasmid # 65417)
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ARHGEF9
  • Alt name
    Collybistin
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Arhgef9
  • Promoter human Synapsin promoter
  • Tag / Fusion Protein
    • HA- in CB2SH3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine was a gift from Angel de Blas (Addgene plasmid # 160069 ; http://n2t.net/addgene:160069 ; RRID:Addgene_160069)

  • For your References section:

    Selective overexpression of Collybistin in mouse hippocampal pyramidal cells enhances GABAergic neurotransmission and protects against PTZ-induced seizures. George S, James S, de Blas AL. eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.2021. doi: 10.1523/ENEURO.0561-20.2021. 10.1523/ENEURO.0561-20.2021 PubMed 34083383
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