pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine
(Plasmid #160069)

• Purpose: Conditionally overexpress collybistin (CB2SH3-) and mCitrine
• Depositing Lab: Angel de Blas
• Publication: George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.2021. doi: 10.1523/ENEURO.0561-20.2021.

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Backbone

• Vector backbone: pAAV2
• Backbone manufacturer: Modified from Bryan Roth (Addgene plasmid # 65417)
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ARHGEF9
• Alt name: Collybistin
• Species: R. norvegicus (rat)
• Entrez Gene: Arhgef9
• Promoter: human Synapsin promoter
• Tag / Fusion Protein:
  • HA- in CB2SH3 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: EcoRV (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry