GFP-RB 1-602
(Plasmid #16006)

• Depositing Lab: Olimpia Meucci
• Publication: Khan et al Cell Death Differ. 2008 Oct . 15(10):1663-72.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rb
• Species: H. sapiens (human)
• Insert Size (bp): 1806
• Mutation: Amino acids 1-602
• Entrez Gene: RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GFP-Rb (1-928) was digested with PstI and SmaI. The 3' overhangs were removed by Klenow treatment and the resulting fragment was self-ligated (after purification).