PB-iCas9
(Plasmid #160048)

• Purpose: inducible expression of Cas9
• Depositing Lab: Xiaojun Lian
• Publication: Lian Lab PB plasmids (unpublished)

Backbone

• Vector backbone: PB
• Total vector size (bp): 3000
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Species: Synthetic
• Insert Size (bp): 4272
• Promoter: TRE

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13-rev

Resource Information

• Supplemental Documents:
  • PB-iCas9.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry