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Purpose(Empty Backbone) For cloning sgRNA to knockout a specific gene in piggybac plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePB
- Backbone size (bp) 3000
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Vector typeMammalian Expression, CRISPR ; piggybac
- Promoter EFS
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-CRISPR was a gift from Xiaojun Lian (Addgene plasmid # 160047 ; http://n2t.net/addgene:160047 ; RRID:Addgene_160047) -
For your References section:
Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems. Jiang Y, Hoenisch RC, Chang Y, Bao X, Cameron CE, Lian XL. Bioact Mater. 2022 Feb 7;14:313-320. doi: 10.1016/j.bioactmat.2022.01.046. eCollection 2022 Aug. 10.1016/j.bioactmat.2022.01.046 PubMed 35386818
