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vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR
(Plasmid #160046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone manufacturer
    SYSTEM BIOSCIENCES
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    osTIR1
  • Species
    Oryza Sativa (Asian rice)
  • Insert Size (bp)
    1725
  • Promoter pEF1s

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGACATACTTTCCTGAAGAGGTCGTC
  • 3′ sequencing primer CAGAATCTTCACAAAGTTGGGAGC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    iaaH
  • Insert Size (bp)
    1401
  • Promoter no promoter, T2A is used

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cttggtaccgagctcggatccaactcgaggccaccatgGTAGCCATCACGTCACTGGCCC
  • 3′ sequencing primer CCACATCCCCGCAAGTAAGCAGTGATCCGCGTCCCTCggtGTTCGGAAGTCCAGCGAAAT
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    iCasp9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1236
  • Promoter no promoter, T2A is used
  • Tags / Fusion Proteins
    • mGFPmut3 (N terminal on insert)
    • mAID (N terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atggctagcaaaggtGAGGAG
  • 3′ sequencing primer gtcgagtgcgtagtctggtacgtcgtacggatag
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    BlastR
  • Alt name
    BSD
  • Species
    Synthetic; Aspergillus terreus
  • Insert Size (bp)
    396
  • Promoter no promoter, T2A is used
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • mAID (N terminal on insert)

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gtgagcaagggcgaggagg
  • 3′ sequencing primer taagctgcaataaacaagttgTCAgccctcccacacataacca
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

osTIR1 is cloned from #72834
mAID is cloned from #72827
iCasp9 is cloned from #15567

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR was a gift from Michael Elowitz (Addgene plasmid # 160046 ; http://n2t.net/addgene:160046 ; RRID:Addgene_160046)

  • For your References section:

    Synthetic mammalian signaling circuits for robust cell population control. Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB. Cell. 2022 Mar 17;185(6):967-979.e12. doi: 10.1016/j.cell.2022.01.026. Epub 2022 Mar 1. 10.1016/j.cell.2022.01.026 PubMed 35235768
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