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vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
(Plasmid #160042)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone manufacturer
    SYSTEM BIOSCIENCE
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    osTIR1
  • Species
    Oryza Sativa (Asian rice)
  • Insert Size (bp)
    1725

Gene/Insert 2

  • Gene/Insert name
    BlastR
  • Alt name
    BSD
  • Insert Size (bp)
    396
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • mAID (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR was a gift from Michael Elowitz (Addgene plasmid # 160042 ; http://n2t.net/addgene:160042 ; RRID:Addgene_160042)

  • For your References section:

    Synthetic mammalian signaling circuits for robust cell population control. Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB. Cell. 2022 Mar 17;185(6):967-979.e12. doi: 10.1016/j.cell.2022.01.026. Epub 2022 Mar 1. 10.1016/j.cell.2022.01.026 PubMed 35235768
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