vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
(Plasmid
#160042)
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PurposeFor the auxin receiver that report on auxin.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160042 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePiggyBac
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Backbone manufacturerSYSTEM BIOSCIENCE
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Vector typeMammalian Expression, Luciferase
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameosTIR1
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SpeciesOryza Sativa (Asian rice)
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Insert Size (bp)1725
Gene/Insert 2
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Gene/Insert nameBlastR
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Alt nameBSD
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Insert Size (bp)396
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Tags
/ Fusion Proteins
- mCherry (N terminal on insert)
- mAID (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe osTIR1 and mAID is obtained from #72834
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR was a gift from Michael Elowitz (Addgene plasmid # 160042 ; http://n2t.net/addgene:160042 ; RRID:Addgene_160042) -
For your References section:
Synthetic mammalian signaling circuits for robust cell population control. Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB. Cell. 2022 Mar 17;185(6):967-979.e12. doi: 10.1016/j.cell.2022.01.026. Epub 2022 Mar 1. 10.1016/j.cell.2022.01.026 PubMed 35235768
