pBMNb-cALFA
(Plasmid #159991)

• Purpose: (Empty Backbone) For the protein expression with ALFA tag at C-terminus in mammalian cells
• Depositing Lab: Motoyuki Hattori
• Publication: Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi: 10.1038/s42003-021-01891-y.

Backbone

• Vector backbone: pFastBac-1
• Vector type: Mammalian Expression
• Tag / Fusion Protein:
  • HRV3C protease cleavage site-ALFA tag-twin Strep tag-8histidine (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Mutation: CMV promoter, WPRE
• Tag / Fusion Protein:
  • HRV3C protease cleavage site-ALFA tag-twin Strep tag-8histidine (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry