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Addgene

mEGFP-NbALFA
(Plasmid #159986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mEGFP-NbALFA
  • Species
    Synthetic
  • Mutation
    3GS linker between mEGFP and NbALFA
  • Tag / Fusion Protein
    • 8histidine-HRV3C protease cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEGFP-NbALFA was a gift from Motoyuki Hattori (Addgene plasmid # 159986 ; http://n2t.net/addgene:159986 ; RRID:Addgene_159986)

  • For your References section:

    Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins. Jin F, Shen C, Wang Y, Wang M, Sun M, Hattori M. Commun Biol. 2021 Mar 19;4(1):366. doi: 10.1038/s42003-021-01891-y. 10.1038/s42003-021-01891-y PubMed 33742097
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