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PurposePrime editing in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePE2-SpRY-P2A-GFP
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SpeciesSynthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
- Promoter CMV
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Tag
/ Fusion Protein
- BpSV40 NLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH-reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.11.10.377580v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-PE2-SpRY-P2A-GFP was a gift from Yongsub Kim (Addgene plasmid # 159981 ; http://n2t.net/addgene:159981 ; RRID:Addgene_159981) -
For your References section:
Engineered Prime Editors with PAM flexibility. Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y. Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 10.1016/j.ymthe.2021.02.022. 10.1016/j.ymthe.2021.02.022 PubMed 33636398
