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Addgene

pCMV-PE2-NG
(Plasmid #159977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2-NG
  • Species
    Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
  • Promoter CMV
  • Tags / Fusion Proteins
    • BpSV40 NLS (N terminal on insert)
    • BpSV40 NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PE2-NG was a gift from Yongsub Kim (Addgene plasmid # 159977 ; http://n2t.net/addgene:159977 ; RRID:Addgene_159977)

  • For your References section:

    Engineered Prime Editors with PAM flexibility. Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y. Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 10.1016/j.ymthe.2021.02.022. 10.1016/j.ymthe.2021.02.022 PubMed 33636398
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