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Addgene

AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE
(Plasmid #159973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 4543
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-YPET-optoNES.V416I
  • Species
    Synthetic
  • Insert Size (bp)
    1250
  • Mutation
    V416I
  • Promoter hSyn1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site multiple; modular (not destroyed)
  • 3′ cloning site multiple; modular (unknown if destroyed)
  • 5′ sequencing primer 3'Syn-fwd
  • 3′ sequencing primer WPRE-
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructs are multipart, modular and generated iteratively.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE was a gift from Michael Courtney (Addgene plasmid # 159973 ; http://n2t.net/addgene:159973 ; RRID:Addgene_159973)

  • For your References section:

    Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators. Li LL, Klein FM, Li Greci L, Popinigis A, Freudenberg F, Courtney MJ. Nat Commun. 2020 Oct 9;11(1):5107. doi: 10.1038/s41467-020-18816-8. 10.1038/s41467-020-18816-8 PubMed 33037199
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