AAV-Synapsin-mCherry-C1-WPRE
(Plasmid #159956)

• Purpose: untargeted marker
• Depositing Lab: Michael Courtney
• Publication: Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi: 10.1038/s41467-020-18816-8.

Backbone

• Vector backbone: AAV
• Backbone manufacturer: Addgene plasmid 127862
• Backbone size w/o insert (bp): 4543
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-C1
• Species: Synthetic
• Insert Size (bp): 750
• Mutation: synthetic
• Promoter: hSyn1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: multiple; modular (not destroyed)
• 3′ cloning site: multiple; modular (unknown if destroyed)
• 5′ sequencing primer: 3'Syn-fwd
• 3′ sequencing primer: WPRE-

Resource Information

• A portion of this plasmid was derived from a plasmid made by: clontech

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructs are multipart, modular and generated iteratively.