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Addgene

pSM1-1-1
(Plasmid #15995)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLUT4
  • Alt name
    glucose transporter 4
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2400
  • Entrez Gene
    Slc2a4 (a.k.a. Glut4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSM1-1-1 was a gift from Morris Birnbaum (Addgene plasmid # 15995 ; http://n2t.net/addgene:15995 ; RRID:Addgene_15995)

  • For your References section:

    Identification of a novel gene encoding an insulin-responsive glucose transporter protein. Birnbaum MJ. Cell. 1989 Apr 21. 57(2):305-15. 10.1016/0092-8674(89)90968-9 PubMed 2649253
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