pCri_L889-antiSH2-OptoMB-Triple
(Plasmid
#159937)
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PurposeOpto-MB bacterial expression
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Depositing Labs
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCri
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameanti-SH2 MB with SS58 insert
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MutationAsLOV2 V416I_G528A_N538E
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCri_L889-antiSH2-OptoMB-Triple was a gift from Jose Avalos & Jared Toettcher (Addgene plasmid # 159937 ; http://n2t.net/addgene:159937 ; RRID:Addgene_159937) -
For your References section:
Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Carrasco-Lopez C, Zhao EM, Gil AA, Alam N, Toettcher JE, Avalos JL. Nat Commun. 2020 Aug 13;11(1):4045. doi: 10.1038/s41467-020-17837-7. 10.1038/s41467-020-17837-7 PubMed 32792484
