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Addgene

Wild-type CoV-2 ORF8
(Plasmid #159933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    2B-T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CoV-2 ORF8 18-121
  • Species
    CoV-2
  • Mutation
    None
  • Entrez Gene
    ORF8 (a.k.a. GU280_gp09)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x Histidine, TEV cleavable (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Wild-type CoV-2 ORF8 was a gift from James Hurley (Addgene plasmid # 159933 ; http://n2t.net/addgene:159933 ; RRID:Addgene_159933)

  • For your References section:

    Structure of SARS-CoV-2 ORF8, a rapidly evolving immune evasion protein. Flower TG, Buffalo CZ, Hooy RM, Allaire M, Ren X, Hurley JH. Proc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2021785118. doi: 10.1073/pnas.2021785118. 10.1073/pnas.2021785118 PubMed 33361333
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