pLenti-5xUASG-iRFP-B3-timestamp-A
(Plasmid
#159925)
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Purposetimestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti2.1
- Backbone size w/o insert (bp) 4750
- Total vector size (bp) 7875
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiRFP-Timestamp A
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Insert Size (bp)4000
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-5xUASG-iRFP-B3-timestamp-A was a gift from Fei Chen (Addgene plasmid # 159925 ; http://n2t.net/addgene:159925 ; RRID:Addgene_159925) -
For your References section:
RNA timestamps identify the age of single molecules in RNA sequencing. Rodriques SG, Chen LM, Liu S, Zhong ED, Scherrer JR, Boyden ES, Chen F. Nat Biotechnol. 2020 Oct 19. pii: 10.1038/s41587-020-0704-z. doi: 10.1038/s41587-020-0704-z. 10.1038/s41587-020-0704-z PubMed 33077959
