pTRE3G-iRFP-B1-timestamp_A
(Plasmid
#159924)
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PurposeTimestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTRE3G
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Backbone manufacturerTakara bio
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiRFP-Timestamp A
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Insert Size (bp)4000
- Promoter TRE3G
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer WPRE-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE3G-iRFP-B1-timestamp_A was a gift from Fei Chen (Addgene plasmid # 159924 ; http://n2t.net/addgene:159924 ; RRID:Addgene_159924) -
For your References section:
RNA timestamps identify the age of single molecules in RNA sequencing. Rodriques SG, Chen LM, Liu S, Zhong ED, Scherrer JR, Boyden ES, Chen F. Nat Biotechnol. 2020 Oct 19. pii: 10.1038/s41587-020-0704-z. doi: 10.1038/s41587-020-0704-z. 10.1038/s41587-020-0704-z PubMed 33077959
