pcDNA3.1-GAVPO
(Plasmid
#159923)
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PurposeGAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAVPO
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Insert Size (bp)1500
- Promoter CMV
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-GAVPO was a gift from Fei Chen (Addgene plasmid # 159923 ; http://n2t.net/addgene:159923 ; RRID:Addgene_159923) -
For your References section:
RNA timestamps identify the age of single molecules in RNA sequencing. Rodriques SG, Chen LM, Liu S, Zhong ED, Scherrer JR, Boyden ES, Chen F. Nat Biotechnol. 2020 Oct 19. pii: 10.1038/s41587-020-0704-z. doi: 10.1038/s41587-020-0704-z. 10.1038/s41587-020-0704-z PubMed 33077959
