pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]
(Plasmid
#159900)
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PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegans
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST R4-R3
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePmlc-1 | mcherry | cbr-tbb-2 UTR
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SpeciesC. elegans (nematode)
- Promoter Pmlc-1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR] was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 159900 ; http://n2t.net/addgene:159900 ; RRID:Addgene_159900) -
For your References section:
Characterizing a strong pan-muscular promoter (Pmlc-1) as a fluorescent co-injection marker to select for single-copy insertions. El Mouridi S, Peng Y, Frokjaer-Jensen C. MicroPubl Biol. 2020 Sep 3;2020. doi: 10.17912/micropub.biology.000302. 10.17912/micropub.biology.000302 PubMed 32908967
