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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePGC1 alpha
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SpeciesM. musculus (mouse)
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Insert Size (bp)3000
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MutationS570A
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Entrez GenePPARGC1A (a.k.a. LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBruce Spiegelman, DFCI
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA Flag PGC1alpha S570A was a gift from Morris Birnbaum (Addgene plasmid # 15988 ; http://n2t.net/addgene:15988 ; RRID:Addgene_15988) -
For your References section:
Akt/PKB regulates hepatic metabolism by directly inhibiting PGC-1alpha transcription coactivator. Li X, Monks B, Ge Q, Birnbaum MJ. Nature. 2007 Jun 21. 447(7147):1012-6. 10.1038/nature05861 PubMed 17554339